Click on image to view larger version.

Figure 4 Fc Receptor Type IIa on Rat Cardiomyocytes

Immunofluorescent staining of isolated rat cardiomyocytes with a rhodamine-conjugated bovine anti-goat secondary antibody after preincubation with polyclonal goat primary antibodies against Fc receptor IIa, Fc receptor IIb, or Fc receptor IIb/c. Immunofluorescence of fibroblasts and rat leukocytes with a rhodamine-conjugated bovine anti-goat secondary antibody after preincubation with polyclonal goat primary antibodies against Fc receptor IIa served as negative and positive control subjects, respectively (counterstaining with 4',6-Diamidino-2-phenyindole, dilactate [DAPI]; representative figures of n = 6 experiments for each antibody).