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Figure 2 The -smooth muscle actin (SMA) immunohistochemistry in reperfused murine infarcts. (A) In control murine hearts, -SMA staining identifies the vascular media (arrowheads). (B) Serial section stained with omission of the primary antibody serves as a negative control. (C) After 72 h of reperfusion, -SMA immunohistochemistry is predominantly localized in spindle-shaped myofibroblasts (arrows). Relatively few coated vessels are noted (arrowheads). (D) After 7 days of reperfusion, the infarct vasculature matures through the recruitment of mural cells by infarct neovessels (arrowheads). Counterstained with eosin (magnification 200x).