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Figure 1 Gating strategy used for the identification of circulating myeloid dendritic cells and plasmacytoid dendritic cell precursors in peripheral blood by flow cytometry. R1 = gating of peripheral blood mononuclear cells according to their forward and side scatter. R2 = exclusion of granulocytes by side scatter, B lymphocytes by CD19-staining, monocytes by CD14-staining, and dead cells by propidium iodide-staining. R3a and R3b = detection of circulating mDC and pDC precursor as the result of their specific BDCA-1 and BDCA-2 staining, respectively.