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Figure 4 Midostaurin increases eNOS mRNA expression in the aorta of SHR and WKY rats. Rats were treated orally with vehicle or midostaurin (+Mido, 75 mg/kg/day) for 5 days. Endothelial nitric oxide synthase mRNA expression in aortas was analyzed with RNase protection assay. Panels A and C show representative gels of an RNase protection assay. Panels B and D show the results of densitometric analyses. Each column (mean ± SEM) represents data obtained with aortas from nine different rats (***p < 0.001 compared vehicle, tested by ANOVA followed by Fisher protected least-significant-difference test). Abbreviations as in Figures 1 and 2.