Click on image to view larger version.

Figure 2 Three-dimensional projection images of thrombi formed in the presence or absence of adenosine diphosphate receptor antagonists. Blood with fluoresceinated platelets was perfused over immobilized collagen type I fibrils for 6 min at the wall shear rate of 1,500 s^–1 in the absence (Control) or presence of MRS2179 (100 µmol/l) or AR-C69931MX (100 nmol/l), as indicated. The platelet thrombi were scanned in the z-axis by confocal microscopy, and the resulting images were projected on planes rotated around the x-axis at 10° intervals (please see the online version of this article for supplemental videos). The figure shows projection images from the top (A), 60° (B), and the front (C). These images are representative of the results obtained in eight separate experiments.