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Figure 2 Characterization of MI and BMT Mouse Hearts

(A) Experimental protocol. The LCA ligation operation was performed on 8-week-old WT mice. Four weeks after LCA ligation, echocardiographic analysis was performed and BMCs were transplanted into the lethally irradiated WT mice. Then, 4 weeks after BMT, the mice were echocardiographically analyzed again and subjected to the treadmill test. (B) Echocardiographic analysis findings before BMT (4 weeks post-operatively) and before the treadmill test (8 weeks post-operatively). (C) Hemodynamic data of the mice 4 weeks after BMT. Light blue, blue, yellow, and red bars represent Sham-WT BMT, MI-WT BMT, Sham-Pres BMT, and MI-Pres BMT, respectively. (D) Morphometric data from the mice 4 weeks after BMT. (E) Hematoxylin-eosin stain of heart section and cross-sectional area of cardiomyocytes at remote area. (F) Cardiac fibrosis in border and remote area with Azan-Mallory staining. BMC = bone marrow cell; HR = heart rate; HW/BW = heart weight to body weight ratio; LCA = left coronary artery; lungW/BW = lung weight-to-body weight ratio; LVSP = left ventricular systolic pressure; MI = myocardial infarction; other abbreviations as in Figure 1.