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Figure 1 MT Prevents Ang II–Induced Apoptosis, 3-NT Formation, and NOX Membrane Translocation in the Hearts of Mice (n 5) That Were Given 1 mg/kg of Ang II at 7 or 24 h Before the Experiments

Apoptosis was detected by terminal transferase dUTP nick end labeling assay (A) and Western blotting activated caspase-3 (B). All 3-nitrotyrosine (3-NT) formation (bands from 30 to 60 kDa) (C and D) and nicotinamide adenine dinucleotide phosphate oxidase (NOX) p47phox membrane translocation (E) were detected by Western blotting assay. *p < 0.05 versus corresponding control. Ang II = angiotensin II; CAT-TG = catalase-overexpressing transgenic; MT = metallothionein; MT-TG = cardiac-specific, metallothionein-overexpressing transgenic; WT = wild type.