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Figure 7 Exercise Prevents Doxorubicin-Induced Cardiac Apoptosis

Effects of doxorubicin (Doxo) (22.5 mg/kg intraperitoneally for 24 h) in sedentary mice and in mice supplied with running wheels for 21 days (n = 10 per group) on (A) protein expression of TRF2 (*p < 0.05 and **p < 0.01 vs. vehicle-treated sedentary control), (B) telomerase activity as determined by telomerase repeat amplification protocol assays, and (C, D) p53 protein expression (quantification and representative Western blots). (E) Quantification of cardiomyocyte apoptosis in C57/Bl6 and (F) in B6.129S TERT^+/+ and TERT^–/– mice by hairpin oligonucleotide assays. *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle-treated sedentary control, +p < 0.05 versus Doxo-treated sedentary control. Abbreviations as in Figures 1 and 5.