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Figure 3 RhoA Activity Is Reduced by AAMP Blockade

(A) The siRNA knockdown reduced AAMP content in the membrane fraction but not in the cytosolic fraction. (B) The RhoA activity assay: in the membrane fraction, siRNA and anti-rAAMP-ab treatment greatly reduced RhoA activity, whereas activated RhoA expression was elevated after AAMP overexpression. In the cytosolic fraction, expression of inactive RhoA was not affected by anti-rAAMP-ab treatment, vector transfection, or AAMP siRNA treatment. Abbreviations as in Figure 2.