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Figure 2 Phenotype-Related AAMP Expression in SMCs and the Effect of AAMP on SMC Migration

(A) Western blot analysis of angio-associated migratory cell protein (AAMP) expression in human smooth muscle cells (hSMCs) and rat smooth muscle cells (rSMCs). (B) Blockade of AAMP by an inhibitory recombinant AAMP-antibody (anti-rAAMP-ab) reduced the migratory potential of medial and of neointimal rSMCs in modified transwell chambers. (C) The migratory activity of hSMCs and rSMCs after AAMP-sense vector-transfection was increased compared with controls. (D) The AAMP knockdown by small interfering ribonucleic acid (siRNA) alleviated hSMC migration compared with controls. (E) Five-bromodeoxyuridine (BrdU) assays showed no effect of anti-rAAMP-ab treatment, vector transfection, and siRNA treatment on SMC proliferation. GFP = green fluorescent protein.