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Figure 6 Growth Factor Expression In Vitro

(A) An increase in vascular endothelial growth factor (Vegf₁₆₅) mRNA production by CD56⁺CD34⁺CD144⁺ cells was observed when cultured under hypoxia for 24 h in comparison with that seen in control normoxic conditions. In contrast, hepatocyte growth factor (Hgf), basic fibroblast growth factor (b-Fgf), and insulin-like growth factor-I (Igf-I) mRNA expression by human CD56⁺CD34⁺CD144⁺ cells either significantly decreased or was not detectable after 24 h of hypoxia. (B) All cell types greatly increased their secretion of Vegf mRNA after exposure to hypoxic culture conditions for 24 h when compared with control cells in normoxia culture conditions by quantitative polymerase chain reaction analysis of Vegf mRNA expression (*p < 0.01, normoxia vs. hypoxia). (C) These results were further confirmed by VEGF ELISA analysis (*p < 0.05, normoxia vs. hypoxia).