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Figure 2 Calcium-Dependent NADPH Oxidase Activity and NOX5 Expression in CAD

(A) Calcium-independent (left) and -dependent (right) NADPH oxidase activity in human coronary arteries in relation to the presence of coronary artery disease (CAD). The NADPH oxidase activity was measured by ESR as described in the Methods section in membranes isolated from coronary arteries of subjects with (n = 8) and without (n = 8) CAD. (B) The NOX5 messenger ribonucleic acid (mRNA) expression in coronary arteries from patients with (n = 13) and without (n = 11) CAD. TaqMan real-time polymerase chain reaction analysis was performed using commercially available gene expression assays. (C) Relationship between Ca⁺⁺-dependent NADPH oxidase activity and NOX5 mRNA expression in studied coronary arteries. Data were expressed as mean ± SEM. *p < 0.05 versus non-CAD. **p < 0.01 versus non-CAD. Abbreviations as in Figure 1.