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Figure 1 Contribution of NOX5 to Calcium-Dependent NADPH Oxidase Activity in Human Endothelial Cells

(A) Example ESR spectra of nitroxide adduct formation by membranes prepared from human aortic endothelial cells (HAECs) in Ca⁺⁺-free media and in the presence of 1 mM Ca⁺⁺ (left) and the effects of small interfering ribonucleic acid (siRNA) against NOX5 on calcium-dependent signal (right). (B) Effects of siRNA NOX5 on NOX5 protein in HAECs; n = 4 experiments. (C) Average Ca⁺⁺-dependent (top) and Ca⁺⁺-independent (bottom) NADPH oxidase activity in the presence of control siRNA (solid bars) and NOX5 siRNA (open bars). n = 4. Values are presented as mean ± SEM. *p < 0.01 versus control siRNA. ESR = electron spin resonance; NADPH = nicotinamide adenine dinucleotide phosphate.