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Figure 1 Confirmation of hNIS-Expression

(A) Immunostaining confirmed the expression of human sodium-iodide symporter (hNIS) (green) in rat cardiac-derived stem cells after lentiviral transduction (nuclei were counterstained by blue Hoechst dye). (B) hNIS messenger ribonucleic acid was detected by reverse transcription-polymerase chain reaction in rat hearts after transplantation of sodium-iodide symporter (NIS)⁺ rat cardiac-derived stem cells (band at 353 base pairs [bp] white arrow, I). The white arrow for II indicates the lack of a specific band in a rat heart injected with the same number of NIS^– cells. (C) Confirmation of hNIS activity in transduced cells. NIS transduction promoted in vitro technetium 99m (pertechnetate) uptake by NIS⁺ rat cardiac-derived stem cells; this uptake was abolished by the specific NIS blocker sodium perchlorate (100 µmol/l) (2 independent experiments, each condition tested in triplicate). ctr = control.