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Figure 8 Gene Expression Data

Gene expression analysis of CD34⁺ cells from 6 anonymous blood donors. (A) Expression of bone-related and other genes normalized to undifferentiated human bone marrow stromal cells (hMSCs; expression of each gene in hMSCs set at 1.0); (B) changes in expression of messenger ribonucleic acids (mRNAs) for CD34 and CD45 following in vitro culture; (C) changes in expression of mRNAs for osteopontin and Col1 alpha₁ following in vitro culture; and (D) changes in mRNA expression for Col1 alpha₁ following in vitro culture in the 3 samples with the highest versus the lowest expression of OCN mRNA prior to culture. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with 1.0 using a 1-sample t test; p < 0.01 and p < 0.001 compared with freshly sorted CD34 cells using a 2-sample t test. N.D. = not detected; other abbreviation as in Figure 1.