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Figure 7 Mineralization Assays

In vitro mineralization assay of CD34⁺ cells from an anonymous blood donor using (A) von Kossa staining and (B) alizarin red staining, and (C) of peripheral blood mononuclear cells from a patient with LCA (alizarin red stain). In these cultures, cells were exposed to Ca²⁺ for 5 days followed by 21 days of culture in osteoblast differentiation medium. All images are at 4x magnification. Abbreviation as in Figure 2.