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Figure 1 Study Design

Protocol 1: Dorsal hand vein infusions of (Pyr¹)apelin-13 or apelin-36 (blue; nmol/min), sodium nitroprusside (SNP) (red; nmol/min) and saline, with coinfusion of norepinephrine. Protocol 2 (A and B): Intrabrachial infusions of (Pyr¹)apelin-13 or apelin-36 (blue; nmol/min) and saline with venous blood sampling (). Protocol 3: Intrabrachial infusions of (Pyr¹)apelin-13 (blue; nmol/min) and acetylcholine (yellow; µg/min) with coadministration of aspirin or placebo, and "nitric oxide clamp" or saline placebo. L-NMMA = L-N ^G-monomethylarginine.