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Figure 7 Immunofluorescent Staining in Noncalcified and Calcified Regions of Stenotic Aortic Valves

Immunofluorescent staining for Msx1 (A and B), Msx2 (C and D), and CBFA1 (E and F) in noncalcified and calcified regions of stenotic aortic valves (inset images are negative control images from adjacent sections; representative images from n = 5/stain). Panels G and H show messenger ribonucleic acid (mRNA) levels of osteopontin and CBFA1/Runx2 mRNA in normal valves and in noncalcified and calcified regions of stenotic aortic valves (*p < 0.05 vs. normal tissue, #p < 0.05 vs. noncalcified stenotic tissue, n = 8/group). Abbreviations as in Figure 1.