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Figure 4 Early EPCs Do Not Incorporate Into Vascular Networks

(A) Early EPCs were pre-tagged with CellTracker 5-(((4-Chloromethyl)benzoyl)amine)tetramethyl rhodamine (red), co-cultured with differentiated ECs, then co-cultures were stained with UEA-1, fluorescein isothiocyanate (FITC) (green), and 4, 6-diamidino-2-phenylindole (blue). (B and C) Three-dimensional z-stacking micrograph showing a tagged early EPC and differentiated EC tubule in separate focal planes (white lines above and right of the images represent position in the z axis). Abbreviations as in Figures 1 and 3.