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Figure 2 2-Dimensional Gel Electrophoresis Map of Atrial Appendages

Protein extracts were separated on a pH 3 to 10 nonlinear immobilized pH gradient strip, followed by separation on a 12% sodium dodecyl sulfate polyacrylamide gel. Spots were detected by silver staining. The figure represents a direct overlay of average gels from hearts in sinus rhythm (SR) and atrial fibrillation (AF) (blue and orange for SR and AF, respectively) created by using the Proteomweaver software (Definiens AG, Munich, Germany). Differentially expressed spots are numbered. Proteins were identified by mass spectrometry and are listed in Table 2.