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Figure 5 Effects of Sirolimus on Mitochondrial ROS Formation

Mitochondrial ROS formation after in vivo sirolimus treatment for 7 days. (A) Increased formation of ROS in response to sirolimus as detected using L012-derived chemiluminescence in isolated mitochondria. *p = 0.020; n = 8 animals control, n = 10 animals sirolimus. (B) Modulation of mitochondrial ROS formation by cyclosporine A (CsA, 0.2 µM), glibenclamide (Gliben., 10 µM), rotenone (Roten., 5 µM), and diazoxide (Diaz., 100 µM). p = 0.005 for Ctr versus Ctr/Diaz., other p values as indicated. n = 5 animals per group. Abbreviations as in Figure 3.