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Figure 1 Effects of Sirolimus on Vascular Function, NO Formation, and Serum Nitrite Levels

Effects of continuous sirolimus (Sir) infusion (5 mg/kg/day for 7 days) on the concentration response relationship to acetylcholine (ACh) (A) and nitroglycerin (NTG) (B) in aortic rings. (C) Electron paramagnetic resonance showing an approximately 40% reduced vascular nitric oxide (NO) formation after ACh stimulation (*p = 0.001; n = 7 rats per group). (D) Decreased serum nitrite levels (–48%; p = 0.018; n = 5 rats per group). Data are the mean ± SEM of n = 5 (A, B), and 2 (C, D) independent experiments. Ctr = control animals.