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Figure 7 Proliferation and Migration Assays

(A) Quantitative data of proliferation assay. Markedly increase proliferation of the smooth muscle cells (SMCs) was observed by stimulation of PDGF-BB (Biosource International, Camarillo, California) compared with those of the native group. The 0.2-µmol/l clarithromycin (CAM) treatment did not significantly inhibit the cell proliferation, whereas 2.0- and 20-µmol/l CAM treatment significantly inhibited the proliferation compared with the control group. *p < 0.05 versus control group. (B) Representative light micrographs of migration assay. Although the SMCs markedly migrated to a lower well by the stimulation of PDGF-BB (b) compared with those in the native group (a), CAM (20 µmol/l) treatment (c) significantly suppressed the SMC migration. (C) The quantitative data of migration assay. Counts of SMCs in a lower well significantly decreased in CAM-treatment group compared with the control group. *p < 0.05 versus control group.