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Figure 6 Western Blot of Phospo-p70S6 Kinase and Total p70S6 Kinase in an In Vitro Study
(Top) Western blot analysis of p70S6 kinase to furthermore confirm the mitochondrial KATP channels on p70S6 kinase activity in homogenates of the left ventricle from the border zone in a rat isolated heart model. P70S6 kinase activity was measured with phospho-specific antibody against phosphorylated p70S6 kinase (top band) and a total p70S6 kinase antibody (bottom band). A significantly increased phospho-p70S6 kinase is noted in the groups treated with a combination of KATP channel agonists and 5-hydroxydecanoate (5-HD) compared with that seen in the groups treated with KATP channel agonists alone. (Bottom) Densitometric quantification of phosphorylation levels was expressed as the ratio of the density of phosphorylated band over total p70S6 kinase. Each point is an average of 3 separate experiments. *p < 0.007 compared with vehicle-, 5-HD-, Nic + 5-HD-, and Pin + 5-HD–treated groups. Abbreviations as in Figure 1.