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Figure 3 PC Immunization Also Raises Anti-oxLDL IgG and IgM

(Left and center) Enzyme-linked immunosorbent assays (ELISA) were performed on sera collected at death to measure IgG and IgM anti-oxidized low-density lipoprotein (oxLDL) titers. Individual sera from each mouse were tested at increasing dilutions (from 1:6.25 to 1:100). Data are represented as mean ± SEM for each group of treatment. Reactivity against native LDL was not different from background signal (data not shown). *p < 0.05 versus phosphate-buffered saline or keyhole lympet hemocyanin (1-way analysis of variance). (Right) Competitive ELISA was performed by coating 2 µg/ml PC-bovine serum albumin (BSA) onto enzyme immunoassay plates and using serum dilutions corresponding to 50% of maximal binding on 2 µg/ml PC-BSA as determined by preliminary experiments. Increasing concentrations of oxLDL were coincubated with the individual sera for 1 h at room temperature. As shown, the level of binding of anti-PC IgG decreased with increasing concentrations of oxLDL, suggesting competition of the 2 antigens for the same antibodies. Abbreviations as in Figures 1 and 2.