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Figure 2 PC Immunization Raises Anti-PC IgG and IgM

Enzyme-linked immunosorbent assays were performed on sera collected at death (similar results were obtained on sera collected by retro-orbital puncture 9 weeks after the first immunization) to measure total immunoglobulin (Ig)G and IgM as well as anti-phosphorylcholine (PC) IgG and IgM titers. Individual sera from each mouse were tested at 1:10 dilution for specific antibodies and at 1:50 dilution for total antibodies. Data are represented as mean ± SEM for each group of treatment. *p < 0.05 versus PBS or KLH (1-way analysis of variance). OD = optical density; other abbreviations as in Figure 1.