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Figure 6 Effects of Rimonabant and AM281 on DOX-Induced Myocardial Apoptosis In Vivo Determined by TUNEL and DNA Fragmentation Assay

(A) Note the increased myocardial terminal deoxynucleotidyltransferase-mediated nick-end labeling (TUNEL) staining from doxorubicin (DOX)-treated mice (brown). Treatment with cannabinoid-1 antagonist rimonabant and AM281 reduced myocardial TUNEL staining in DOX-treated mice. Similar immunohistochemical profiles were seen in n = 3 hearts per group. Quantitative measurements were carried out in 20 fields per group. *p < 0.05 versus vehicle (Veh); #p < 0.05 versus DOX. (B) Effects of rimonabant and AM281 on DOX-induced myocardial apoptosis in vivo by TUNEL assay. *p < 0.05 versus vehicle; #p < 0.05 versus DOX (n = 4 per group). (C) Effects of rimonabant and AM281 on DOX-induced deoxyribonucleic acid (DNA) fragmentation in vivo. Changes are expressed in % of myocardial DNA fragmentation in vehicle-treated mice (100%). *p < 0.05 versus vehicle; #p < 0.05 versus DOX (n = 4 per group). Eu = Europium.