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Figure 6 Apoptotic Cardiomyocyte Cell Death in Hamster Hearts 14 Weeks After Gene Transfer

(A) Images of apoptotic cardiomyocytes. Triple staining with propidium iodide, terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL), and anti--sarcomeric actin antibody. (B) Number of TUNEL-positive cardiomyocytes. *p < 0.05 versus rAAV/LacZ (n = 5). (C) Immunohistochemical staining of activated caspase 3 (left panels). Normal rabbit immunoglobulin (Ig)G was used as a negative control (right panels). Arrows indicate activated caspase 3-positive cells. (D) Number of activated caspase 3-positive cardiomyocytes. *p < 0.05 versus rAAV/LacZ (n = 5). Abbreviations as in Figure 1.