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Figure 2 Gel Electrophoresis of PCR Products

(A) Polymerase chain reaction (PCR) products generated by nested reverse transcriptase (RT)-PCR using primers located in the 5'-noncoding region of the enterovirus genome (360 bp). Lanes 1 to 4 correspond to patients who died of an inaugural acute myocardial infarction (MI); lanes 5 to 8 correspond to patients with a previous history of chronic coronary disease and who died of an acute MI; P1 = positive control obtained by RT-PCR amplification of positive ribonucleic acid (RNA) strands (2 x 10¹⁰ copies), which were transcribed from a coxsackievirus B3 (CV-B3) complementary (c) deoxyribonucleic acid (DNA) sequence; P2 = positive control obtained by direct PCR amplification of single-strand copies of CV-B3 cDNA; NC1 = negative control of RT assay and nested PCR amplification; NC2 = negative control (sterile water) of the first PCR amplification assay; NC3 = negative control of the second PCR amplification assay; M = molecular size marker (100-bp DNA ladder). (B) PCR products generated by RT-PCR using primers located in the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) housekeeping gene (190 bp). Lanes 1 to 8 correspond to a positive GAPDH messenger RNA detection in cardiac tissues from patients 1 to 8. NC4 = RT-PCR negative control (sterile water); M = molecular size marker (100-bp DNA ladder).