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Figure 2 Cell Differentiation and Myotube Formation

Light photomicrographs (100x) showing cultured cells derived from young and older rats, stained for myosin heavy chain (MHC) and desmin at passages 1 (P1) (A to D) and 5 (P5) (E to H). (I) The number of myotubes in cell cultures derived from young and older rats at passages 1 and 5. *p = 0.01 compared with same group at P1.