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Figure 3 Hypoxia Induces the Endoplasmic Reticulum Stress Response Genes in HL1 Cardiomyocytes, and Zinc-Superoxide Dismutase-1 Is a Target of the Anti-Apoptotic Action of PDI

(A) Total RNA of hypoxia-treated murine HL1 cells was analyzed by quantitative reverse transcriptase polymerase chain reaction for the expression levels of PDI, Ero1, Grp94, binding protein (BIP), and the transcription factors PERK, IRE1-alpha, and ATF6-alpha. *Significant differential expression (Student t test, n = 4, p < 0.05) after exposure to hypoxia. (B) Hydrogen peroxide (H₂O₂) release was measured in HL1 cells by horseradish peroxidase-enhanced chemiluminescence. Given are the mean ± SD from 3 independent measurements. *Significant differential expression (Student t test, n = 5, p < 0.05). Abbreviations as in Figure 1.