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Figure 6 Everolimus-Induced Autophagy in Cultured Macrophages But Not in SMC

(A) Ultrastructural features of a normal mouse macrophage as an untreated control with normal cell morphology. (B to D) Treatment of macrophages with everolimus (10 µmol/l) showing different stages of autophagic cell death, which was characterized by cell shrinkage, extensive vacuolization (*), depletion of organelles, and presence of an intact, nonpyknotic nucleus (N). (E) Ultrastructural features of a normal mouse smooth muscle cells (SMC) as an untreated control. (F) Autophagy was not induced in everolimus-treated (10 µmol/l, 6 h) SMC. Scale bar = 3 µm.