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Figure 4 Gene-Silencing Experiments With mTOR-Specific siRNA

(A) Downregulation of mTOR gene expression at the mRNA level in both macrophages (M) and smooth muscle cells (SMC) 24 h after transfection with mTOR-specific siRNA but not with siControl nontargeting siRNA. (B) Downregulation of mTOR gene expression at the protein level in both M and SMC after transfection with mTOR-specific siRNA but not with siControl nontargeting siRNA. (C) mTOR gene silencing induces macrophage cell death of M but not of SMC. Versus control, unpaired Student t test: **p < 0.01; ***p < 0.001. mTOR = mammalian target of rapamycin.