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Figure 3 In Vitro Effect of Everolimus, Tacrolimus, and Cycloheximide on the Viability of Cultured Mouse M and SMC

(A) Cells were exposed to 10 µmol/l everolimus (0 to 24 h). (B) Cells were exposed to 1 to 10 µmol/l everolimus for 24 h. Neutral red uptake showed that viability was preserved exclusively to SMC. In contrast, macrophages underwent cell death. (C) Cells were exposed to 10 µmol/l tacrolimus (0 to 24 h). (D) Cells were exposed to 1 to 10 µmol/l tacrolimus for 24 h. Neutral red uptake showed that tacrolimus did not induce cell death in M and SMC. (E, F) Similar to everolimus, cycloheximide (10 µg/ml) also induced rapid cell death of macrophages, whereas the effect on viability of SMC was limited. Versus control: *p < 0.05; **p < 0.01; ***p < 0.001. Open bars = M; shaded bars = SMC. Abbreviations as in Figure 1.