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Figure 5 LPS- or Ang II-Induced TUNEL Staining of Cardiac Myocytes Inhibited by BAPTA-AM or Thapsigargin

Terminal deoxy-nucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining of cardiac myocytes (mean % + SE) increased after 24 h with lipopolysaccharide (LPS) (10 ng/ml) or angiotensin II (Ang II) (100 nmol/l) compared with control (vehicle) (p < 0.05). This was blocked by pre-treating myocytes 1 h before LPS or Ang II with: (A) 1,2-Bis(2-amino-5-fluorophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl) ester (BAPTA-AM) (0.1 µmol/l), while BAPTA-AM alone had no effect (n = 5); (B) Thapsigargin (TG) (1 µmol/l), while TG alone had no effect (n = 9).