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Figure 6 Effect of H₂O₂ and L-cysteine on CD40 Glycoprotein Expression in HUVECs

(A) The upper left panel shows untreated cells (the open histogram represents the irrelevant antibody, whereas the blue histogram depicts the anti-CD40 antibody). The upper right panel shows a comparison between untreated cells (blue histogram) and cells treated with H₂O₂ (300 µM) for 30 min (open histogram). Cells were washed and cultured in normal medium for 24 h before flow cytometric analysis (upper panels). The graph in the lower panel shows mean ± SD from 3 different experiments. (B) The upper left panel shows untreated cells (the open histogram represents the irrelevant antibody, whereas the blue histogram depicts the anti-CD40 antibody). The upper right panel shows a comparison between untreated cells (blue histogram) and cells treated with L-cysteine (500 µM) for 12 h. The lower panel shows mean ± SD from 3 separate experiments. Ctrl = control; other abbreviations as in Figure 3.