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Figure 2 Inflammatory Response According to the NF-B Activation Status (Protocol A)

Serum levels of CRP (A), and production of IL-6 (B) and TNF- (C) in response to in vitro LPS challenge were significantly higher in patients with (NF-B+) than in those without (NF-B–) NF-B activation. (B and C) Monocytes (5 x 10⁵) were stimulated with a low dose of LPS (1 ng/ml); after 4 h of incubation, the culture supernatant was removed and stored at –80°C for IL-6 and TNF- measurements by ELISA. CRP = C-reactive protein; IL = interleukin; TNF = tumor necrosis factor; other abbreviations as in Figure 1.