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Figure 1 Percutaneous Model of Hibernating Myocardium

After percutaneous implantation of a reduction stent in the proximal left anterior descending artery (LAD) (day 0, arrow) the formation of a high-grade stenosis was angiographically confirmed at day 10 (top). At day 28, (sub)total occlusion of the LAD was noted with distal filling of the LAD through collaterals. At this time point, retroinfusion of liposomes with either green fluorescent protein (GFP) or endothelial nitric oxide synthase (eNOS) S1177D was performed. At day 49, hemodynamic measurements were performed, and tissue was obtained for molecular analysis.