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Figure 8 (A) Representative photomicrographs of hematoxylin-eosin staining of arterial sections 28 days after carotid ligation in mice receiving naloxone (right panel) or phosphate-buffered saline (PBS) injection (left panel) . Arrows indicate borders of the neointima and media. Original magnification 200x. (B) Morphometric analysis of the lumen, neointima, media, and total vascular areas. Values are mean ± SD of 5 sections in each mouse. *p < 0.05; **p < 0.01; ***p < 0.005 compared with the mice receiving PBS injection. (C) Degree of neointima formation 28 days after carotid ligation was calculated according to neointima/media area (N/M) ratio. ***p < 0.005 compared with the mice receiving PBS injection (bar = 100 µm).