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Figure 5 Superoxide formation in the mouse aorta evaluated by oxidative fluorescent microtopography with the oxidative fluorescent dye dihydroethidium (DHE). Representative photomicrographs of the aortic sections in mice receiving lipopolysaccharide (LPS) (A) , 20 mg/kg naloxone pretreatment 1 h before LPS (B) , and phosphate-buffered saline (C) are shown. Increased superoxide production was visualized by amplified red fluorescence in the aortic wall after LPS stimulation (A) . In comparison, staining was much more reduced in the aorta of animals receiving naloxone pretreatment (B) (bar = 50 µm).