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Figure 4 Effect of naloxone treatment on the plasma levels of TNF- (A) and the pulmonary inflammatory cell infiltration (B) in mice. (A) Mice were pretreated with the indicated dose of naloxone (n = 8 in 10 mg/kg, n = 7 in 20 mg/kg, and n = 5 in 25 mg/kg naloxone) or PBS (n = 7) before stimulation with LPS. Plasma TNF- levels were measured by enzyme-linked immunosorbent assay. Data are expressed as mean values ± SD. *p < 0.05; **p < 0.01 compared with the LPS-treated only group (n = 9). (B) Mice were pretreated with the indicated dose of naloxone (n = 5 in 10 mg/kg, n = 6 in 20 mg/kg, and n = 5 in 25 mg/kg naloxone) or PBS (n = 9) before stimulation with LPS. The presence of inflammatory cells in lungs was determined by immunofluorescence. Data are expressed as the mean values ± SD. **p < 0.01 compared with the LPS-treated only group (n = 7). Other abbreviations as in Figure 1 .