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Figure 4 Diabetes-induced cardiac protein nitration and lipid peroxidation. Panel A presents the double staining for cardiomyocytes by -sarcomeric actin (labeled by "-SA") and protein nitration by 3-NT. These images in panel A are the representatives of each group including more than 3 mice with 2 sections from each mouse. For panel B, cardiac tissues were collected from control and diabetic mice (at lease 5 mice for each group) 14 days after STZ treatment, and lipid peroxidation was measured by thiobarbituric acid-reactive substance (TBARS) assay. Data were presented as mean values ± SD. A 2-tailed unpaired Student t test was used. *p < 0.05 versus corresponding control. Abbreviations as in Figure 1.