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Figure 3 Mitochondrial cytochrome c release to cytosol was measured by Western blotting assay in the cytosolic and mitochondrial fractions from the hearts of control and diabetic mice 14 days after STZ treatment. Gel was a representative of 2 experiments using 3 hearts as 1 sample to isolate the cytosolic and mitochondrial proteins. A 2-tailed unpaired Student t test was used. *p < 0.05 versus corresponding control. Abbreviations as in Figure 1.