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Figure 3 Photomicrographs showing staining and localization of neutrophil-activating peptide-2, CXCR1, and CXCR2 in plaque sections of left carotid artery. Plaque sections stained with omission of the primary antibody as control demonstrated no immunostaining of any of the cellular elements (A). Fairly strong immunostaining of neutrophil-activating peptide-2 (B), CXCR1 (C), and CXCR2 (D) was co-localized to areas with calprotecin-positive macrophages (E). No immunostaining was seen in sections from non-atherosclerotic renal arteries when staining for neutrophil-activating peptide-2, CXCR1, or CXCR2 (F). Original magnification x 400.