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Figure 5 Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) in the transition to heart failure. Sheep lymph node tissue used as (a) positive control and (b) negative control with TdT enzyme omitted. Biopsy samples (n = 4 from each stage) were taken at echocardiographically distinct stages of (c) normal left ventricle (control), (d) left ventricular hypertrophy, (e) left ventricular dilation, and (f) left ventricular failure and subjected to peroxidase labeled TUNEL assay. The TUNEL-positivity is shown by dark brown staining nuclei (magnification x20).