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Figure 4 (A) Wild-type (WT) and harlequin (Hq) hearts, subjected to 28 days of mechanical load, displayed increased myocyte apoptosis as scored by cleaved caspase-3–positive myocytes. The percentage increase in Hq mutants was more pronounced and was attenuated by treatment with EUK-8. (B) The WT and Hq hearts demonstrated increased cleaved PARP-positive myocytes after transverse aortic constriction (TAC), with the percentage increase in Hq mutants being more pronounced than in WT. Treatment with EUK-8 significantly reduced the amount of PARP-positive myocytes in Hq-TAC hearts.