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Figure 3 Representative example of mitochondrial permeability transition induced by calcium overload in preparation of isolated mitochondria. Recordings are displayed in one sham (a), one cecal ligation and puncture (CLP) (b), one cyclosporine-A–treated CLP (c), one N-methyl-4-isoleucine cyclosporine (NIM811)-treated CLP (d), and one CLP transgenic mouse (e). In sham mice (a), a Ca²⁺ load of 260 µmol/l (26 pulses of 10 µmol/l Ca²⁺) was required to induce mitochondrial permeability transition versus 80 µmol/l Ca²⁺ (8 pulses of 10 µmol/l Ca²⁺) in wild-type CLP mice.