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Figure 2 Quantitation of endothelial progenitor cells by flow cytometry. Frequency of CD34⁺ and Flk-1⁺ cells in control animals without ischemia, with ischemia, and with ischemia and nicotine treatment (A). Histogram showing number of endothelial progenitor cells in the bone marrow at increasing intervals after induction of ischemia and initiation of oral nicotine (B). For each group, n = 5. *p < 0.01 versus control for each respective time point.