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Figure 4 Activation of the platelet-derived growth factor receptor (PDGFR)-ß signaling pathway in healing murine myocardial infarcts was detected using immunohistochemical staining with an antibody to phosphorylated PDGFR-ß (pPDGFR-ß). (A) Noninfarcted areas showed minimal staining for pPDGFR-ß. (B) After 7 days of reperfusion, perivascular and mononuclear-like cells (arrowheads) with intense immunoreactivity to pPDGFR-ß were found in the infarcted myocardium. (C) Negative control with omission of the primary antibody shows no staining. Counterstained with eosin (magnification 200x).