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Figure 3 Examples of immunohistochemical staining results in cardiac ganglia and nerves. (A) Growth-associated protein 43 (GAP 43) demonstrating axonal growth within the ganglion; (B) synaptophysin (SYN) staining demonstrating synaptic endings; (C) neurofilament (NF) staining confirming the presence of nerve fibers; (D) choline acetyltransferase (ChAT) staining showing cholinergic nature of most ganglion cells; (E) tyrosine hydroxylase (TH) staining showing co-localization of adrenergic cells within the same ganglion; and (F) ChAT positivity of nerve adjacent to ganglion.